{"id":3995,"date":"2023-03-27T12:05:32","date_gmt":"2023-03-27T16:05:32","guid":{"rendered":"https:\/\/neoaptamers.com\/?p=3995"},"modified":"2026-04-13T15:21:24","modified_gmt":"2026-04-13T19:21:24","slug":"dna-vs-rna-aptamers","status":"publish","type":"post","link":"https:\/\/neoaptamers.com\/fr\/dna-vs-rna-aptamers\/","title":{"rendered":"ADN vs. Aptam\u00e8res ARN"},"content":{"rendered":"<p><span style=\"font-weight: 400;\">Dans notre <a href=\"https:\/\/neoaptamers.com\/fr\/what-is-aptamers\/\">\"Qu'est-ce qu'un aptam\u00e8re\"<\/a> dans notre blog, nous avons d\u00e9crit que les aptam\u00e8res sont des mol\u00e9cules d'ADN ou d'ARN synth\u00e9tiquement fabriqu\u00e9es, simples brins. Il existe des diff\u00e9rences inh\u00e9rentes entre l'ADN et l'ARN lorsqu'ils sont trouv\u00e9s naturellement. L'ADN est typiquement une biomol\u00e9cule double brin compos\u00e9e de bases A, T, C et G. L'ARN est simple brin et compos\u00e9 de bases A, U, C et G qui ne sont pas aussi stables dans la cellule car il agit g\u00e9n\u00e9ralement comme un messager pour d'autres syst\u00e8mes.\u00a0<\/span><\/p>\n<h2><\/h2>\n<h2>Choisir entre les aptam\u00e8res d'ADN et d'ARN<\/h2>\n<h2><img fetchpriority=\"high\" decoding=\"async\" class=\"alignnone wp-image-3996 size-full\" style=\"font-size: 16px;\" src=\"https:\/\/neoaptamers.com\/wp-content\/uploads\/2023\/03\/DNAvsRNAAptamers.png\" alt=\"A table demonstrating the structures of DNA and RNA aptamers\" width=\"1365\" height=\"191\" srcset=\"https:\/\/neoaptamers.com\/wp-content\/uploads\/2023\/03\/DNAvsRNAAptamers.png 1365w, https:\/\/neoaptamers.com\/wp-content\/uploads\/2023\/03\/DNAvsRNAAptamers-300x42.png 300w, https:\/\/neoaptamers.com\/wp-content\/uploads\/2023\/03\/DNAvsRNAAptamers-1024x143.png 1024w, https:\/\/neoaptamers.com\/wp-content\/uploads\/2023\/03\/DNAvsRNAAptamers-768x107.png 768w\" sizes=\"(max-width: 1365px) 100vw, 1365px\" \/><\/h2>\n<p><span style=\"font-weight: 400;\">Lors de la conception d'une strat\u00e9gie de s\u00e9lection pour un aptam\u00e8re se liant \u00e0 une cible<\/span>, les diff\u00e9rences inn\u00e9es et leurs avantages et inconv\u00e9nients associ\u00e9s doivent \u00eatre pris en compte lors de la conception d'une strat\u00e9gie de s\u00e9lection pour un aptam\u00e8re se liant \u00e0 une cible. La premi\u00e8re chose \u00e0 d\u00e9cider serait l'application de l'aptam\u00e8re. Cela s'explique par le fait que, comme mentionn\u00e9 ci-dessus, l'ARN agit naturellement comme un syst\u00e8me messager pour la cellule, ce qui signifie que l'ARN natif est destin\u00e9 \u00e0 \u00eatre d\u00e9grad\u00e9 en quelques secondes dans le plasma. L'ADN natif a une demi-vie plus longue dans le plasma, plus proche de 60 minutes. Il existe des modifications chimiques qui peuvent \u00eatre apport\u00e9es \u00e0 l'ARN, comme la substitution d'un fluor \u00e0 la place du groupe hydroxyle naturel \u00e0 la position 2\u2019 sur les bases d'ARN. Cette modification, ainsi que d'autres, peut augmenter la demi-vie de l'ARN pour correspondre, voire d\u00e9passer, celle de l'ADN natif. Cela signifie que si un aptam\u00e8re doit \u00eatre sans modifications dans votre application, alors l'ADN serait pr\u00e9f\u00e9rable en raison d'une meilleure stabilit\u00e9.<\/p>\n<table>\n<tbody>\n<tr>\n<td><\/td>\n<td><i><span style=\"font-weight: 400;\">ADN<\/span><\/i><\/td>\n<td><i><span style=\"font-weight: 400;\">ARN<\/span><\/i><\/td>\n<\/tr>\n<tr>\n<td><i><span style=\"font-weight: 400;\">Stabilit\u00e9 naturelle dans le plasma<\/span><\/i><\/td>\n<td><span style=\"font-weight: 400;\">60 \u00e0 90 minutes<\/span><\/td>\n<td><span style=\"font-weight: 400;\">Secondes<\/span><\/td>\n<\/tr>\n<tr>\n<td><i><span style=\"font-weight: 400;\">Diversit\u00e9 structurale de la biblioth\u00e8que<\/span><\/i><\/td>\n<td><span style=\"font-weight: 400;\">Grande diversit\u00e9 structurale\u00a0<\/span><\/td>\n<td><span style=\"font-weight: 400;\">Grande diversit\u00e9 structurale<\/span><\/td>\n<\/tr>\n<tr>\n<td><i><span style=\"font-weight: 400;\">Diversit\u00e9 structurale des s\u00e9quences\u00a0<\/span><\/i><\/td>\n<td><span style=\"font-weight: 400;\">Des structures plus stables, donc des liaisons plus fiables<\/span><\/td>\n<td><span style=\"font-weight: 400;\">Une plus grande diversit\u00e9 structurelle au sein d'une s\u00e9quence peut entra\u00eener une liaison faible.<\/span><\/td>\n<\/tr>\n<tr>\n<td><i><span style=\"font-weight: 400;\">Temps de s\u00e9lection<\/span><\/i><\/td>\n<td><span style=\"font-weight: 400;\">Court (compar\u00e9 \u00e0 l'ARN)<\/span><\/td>\n<td><span style=\"font-weight: 400;\">Long (n\u00e9cessite une transcription apr\u00e8s chaque tour)<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<h3>Diff\u00e9rences structurelles<\/h3>\n<p><span style=\"font-weight: 400;\">La seule diff\u00e9rence structurelle entre le squelette de l'ARN monocat\u00e9naire et celui de l'ADN monocat\u00e9naire est le groupe hydroxyle en position 5' sur l'ARN. La pr\u00e9sence de ce groupe pourrait entra\u00eener une certaine g\u00eane st\u00e9rique et donc moins de flexibilit\u00e9 au sein de la mol\u00e9cule d'ARN. Bien que plus de structures aient \u00e9t\u00e9 caract\u00e9ris\u00e9es dans l'ARN, c'est parce que l'ARN a \u00e9t\u00e9 \u00e9tudi\u00e9 de mani\u00e8re plus approfondie. En raison de cela, on ne peut pas exclure que l'un ou l'autre ait une plus grande diversit\u00e9 structurelle.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">En revanche, l'ARN a tendance \u00e0 pr\u00e9senter un plus grand nombre de conformations au sein d'une s\u00e9quence, dont seules quelques-unes peuvent participer \u00e0 la liaison \u00e0 la cible. Pendant ce temps, l'ADN a tendance \u00e0 former des structures secondaires plus stables qui sont disponibles pour se lier \u00e0 la cible.\u00a0<\/span><\/p>\n<p>&nbsp;<\/p>\n<h3>Temps de S\u00e9lection<\/h3>\n<p><span style=\"font-weight: 400;\">Lorsque l'on cherche \u00e0 effectuer une s\u00e9lection pour une cible, il faut \u00e9galement prendre en compte le temps n\u00e9cessaire pour effectuer cette s\u00e9lection. Bien que cela semble \u00eatre un probl\u00e8me de moindre importance par rapport aux consid\u00e9rations pr\u00e9c\u00e9demment mentionn\u00e9es, il existe de grandes diff\u00e9rences entre les deux. Une s\u00e9lection avec l'ARN prendra plus de temps car il y a une \u00e9tape suppl\u00e9mentaire de conversion de la biblioth\u00e8que d'ARN en ADN pour permettre son amplification pour les tours suivants. Ensuite, elle doit \u00eatre convertie de nouveau en ARN pour le tour de s\u00e9lection suivant. Cela rend la s\u00e9lection plus longue que celle de l'ADN, o\u00f9 la biblioth\u00e8que doit simplement \u00eatre amplifi\u00e9e entre les tours. Chez NeoVentures, ce temps de s\u00e9lection est consid\u00e9rablement r\u00e9duit gr\u00e2ce \u00e0 notre plateforme Neomers. Avec cette nouvelle m\u00e9thode de s\u00e9lection reproductible des aptam\u00e8res, nous pouvons identifier des aptam\u00e8res hautement sp\u00e9cifiques en une seule \u00e9tape de s\u00e9lection. Cela signifie que le temps n\u00e9cessaire pour la s\u00e9lection est consid\u00e9rablement r\u00e9duit pour les biblioth\u00e8ques d'ARN et d'ADN.\u00a0<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-weight: 400;\">Le succ\u00e8s d'une s\u00e9lection d'aptam\u00e8res d\u00e9pend \u00e9galement fortement de la prise en compte des s\u00e9lections positives et n\u00e9gatives. Chez NeoVentures, nous avons la plus grande exp\u00e9rience dans la conception de la meilleure strat\u00e9gie qui fonctionnera pour votre cible et votre application. <a href=\"https:\/\/neoaptamers.com\/fr\/contact-us\/\">Contactez notre \u00e9quipe pour en savoir plus sur celui que vous devriez utiliser pour votre application unique.<\/a><\/span><\/p>","protected":false},"excerpt":{"rendered":"<p>In our &#8220;What is an aptamer&#8221; blog, we described that aptamers are synthetically made, single-stranded DNA or RNA molecules. There are inherent differences between DNA and RNA when found naturally. DNA is typically a double-stranded biomolecule composed of A, T, C, and G bases. RNA is single-stranded and composed of A, U, C, and G&hellip;&nbsp;<a href=\"https:\/\/neoaptamers.com\/fr\/dna-vs-rna-aptamers\/\" rel=\"bookmark\">Lire la suite &raquo;<span class=\"screen-reader-text\">ADN vs. Aptam\u00e8res ARN<\/span><\/a><\/p>","protected":false},"author":2,"featured_media":3998,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"neve_meta_sidebar":"","neve_meta_container":"","neve_meta_enable_content_width":"off","neve_meta_content_width":70,"neve_meta_title_alignment":"","neve_meta_author_avatar":"","neve_post_elements_order":"","neve_meta_disable_header":"","neve_meta_disable_footer":"","neve_meta_disable_title":"","footnotes":""},"categories":[8,11],"tags":[],"class_list":["post-3995","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-aptamers","category-aptamers-in-diagnostics"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>DNA vs RNA Aptamers | Learn More | neoaptamers<\/title>\n<meta name=\"description\" content=\"DNA vs RNA aptamers for your diagnostic application is key to its success. 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He has used this blend of biology and mathematics to first develop and lead a cereal biotechnology research team with the government of Canada and subsequently as a global research leader with Monsanto Inc. He has been a thought leader in aptamer development globally for the last twenty years as CEO and President of NeoVentures. \u00a0He has led this company to financial stability without outside investment with an integrated approach to aptamer discovery and commercialization. In 2015, he co- founded a second company, NeoNeuro in Paris France, focused on an innovative approach to identify Aptamarkers for complex diseases. Connect with Dr. Penner on LinkedIn or for company updates, follow NeoVentures. Click here to get in touch with our team.\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"DNA vs RNA Aptamers | Learn More | neoaptamers","description":"DNA vs RNA aptamers for your diagnostic application is key to its success. 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