{"id":3101,"date":"2022-11-16T14:19:47","date_gmt":"2022-11-16T19:19:47","guid":{"rendered":"https:\/\/neoaptamers.com\/?p=3101"},"modified":"2022-11-24T22:05:47","modified_gmt":"2022-11-25T03:05:47","slug":"avoiding-self-recognition-with-aptamers","status":"publish","type":"post","link":"https:\/\/neoaptamers.com\/fr\/avoiding-self-recognition-with-aptamers\/","title":{"rendered":"Comment nous \u00e9vitons l'autoreconnaissance avec les aptam\u00e8res"},"content":{"rendered":"<h2>Antibody Binding vs. Aptamer Binding<\/h2>\n<p><span style=\"font-weight: 400;\">La r\u00e9ponse immune s'est d\u00e9velopp\u00e9e de mani\u00e8re tr\u00e8s<\/span><span style=\"font-weight: 400;\">\u00a0les m\u00e9thodes de d\u00e9pistage efficaces pour \u00e9liminer les anticorps qui se lient m\u00eame faiblement \u00e0 des cibles auto comme l'albumine s\u00e9rique et l'IgG. La s\u00e9lection des aptam\u00e8res est r\u00e9alis\u00e9e en dehors du corps, sur la paillasse, ce qui<\/span><span style=\"font-weight: 400;\">fournit de nombreux avantages mais la tol\u00e9rance immunitaire implicite dans la s\u00e9lection n'en fait pas partie.\u00a0<\/span><\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-3102 aligncenter\" src=\"https:\/\/neoaptamers.com\/wp-content\/uploads\/2022\/11\/Diagram.png\" alt=\"Diagram describing self-recognition in aptamers\" width=\"566\" height=\"473\" \/><\/p>\n<p>&nbsp;<\/p>\n<h2>Contre-s\u00e9lection<\/h2>\n<p><span style=\"font-weight: 400;\">La contre-s\u00e9lection contre les hors-cibles a \u00e9t\u00e9 la seule fa\u00e7on de produire des aptam\u00e8res qui ne se lieront pas \u00e0 d'autres cibles dans les fluides corporels. Le SELEX est bas\u00e9 sur l'utilisation de 1E15 s\u00e9quences d\u00e9riv\u00e9es de 1,2E24 s\u00e9quences possibles. Cela signifie que chaque s\u00e9lection est bas\u00e9e sur un nouvel ensemble de s\u00e9quences. Il n'a pas \u00e9t\u00e9 possible de tester le m\u00eame ensemble de s\u00e9quences de d\u00e9part sur diff\u00e9rentes cibles.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\">La contre-s\u00e9lection est tr\u00e8s efficace contre les s\u00e9quences qui se lient \u00e9troitement \u00e0 la hors-cible. Elle n'est pas efficace pour \u00e9liminer les s\u00e9quences qui se lient faiblement. Pour \u00eatre clair, la contre-s\u00e9lection n'est pas aussi efficace ni aussi compl\u00e8te que la tol\u00e9rance immunitaire.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\">Cela a-t-il vraiment de l'importance ? Malheureusement, oui. Les prot\u00e9ines abondantes comme l'albumine s\u00e9rique sont pr\u00e9sentes dans le sang \u00e0 une concentration moyenne de 600 uM. Si nous essayons de d\u00e9tecter des cibles \u00e0 des concentrations beaucoup plus faibles, m\u00eame une contre-liaison faible saturera la liaison des aptam\u00e8res \u00e0 l'albumine s\u00e9rique.\u00a0<\/span><\/p>\n<h2>Notre solution<\/h2>\n<p><span style=\"font-weight: 400;\">NeoVentures a surmont\u00e9 ce probl\u00e8me avec notre nouvelle <a href=\"https:\/\/neoaptamers.com\/fr\/neomers\/\">m\u00e9thode de s\u00e9lection d'aptam\u00e8res Neomer.<\/a>La m\u00e9thode implique l'application en moyenne de 1 000 copies de chacune des m\u00eames 4,29 milliards de s\u00e9quences \u00e0 chaque cible. Cela signifie que nous pouvons identifier toutes les s\u00e9quences qui se lient m\u00eame faiblement aux prot\u00e9ines abondantes et les \u00e9liminer en tant que candidats pour votre cible. En essence, nous avons cr\u00e9\u00e9 une alternative in silico \u00e0 l'\u00e9vitement de l'auto-reconnaissance. Il s'agit d'une innovation r\u00e9volutionnaire qui entra\u00eenera certainement de meilleures performances des aptam\u00e8res dans les applications de diagnostic. <a href=\"https:\/\/neoaptamers.com\/fr\/category\/neomers\/\">Lisez-en plus sur Neomer dans notre blog.<\/a><\/span><\/p>\n<p><span style=\"font-weight: 400;\">Pour en savoir plus sur ce que cela signifie pour votre capacit\u00e9 \u00e0 utiliser des aptam\u00e8res pour surmonter les probl\u00e8mes dans votre portefeuille de diagnostics, <a href=\"https:\/\/neoaptamers.com\/fr\/contact-us\/\">contactez notre \u00e9quipe d\u00e8s aujourd'hui.<\/a>\u00a0<\/span><\/p>\n<p><a href=\"https:\/\/www.linkedin.com\/company\/5348393\/\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: 400;\"> Suivez-nous sur LinkedIn pour \u00eatre inform\u00e9 \u00e0 chaque fois que nous publions de nouvelles informations sur Neomer.<\/span><\/a><\/p>","protected":false},"excerpt":{"rendered":"<p>Antibody Binding vs. Aptamer Binding The immune system has developed very\u00a0effective screening methods for eliminating antibodies that bind even weakly to self targets such as serum albumin and IgG. Aptamer selection is done outside the body, on the bench which provides many advantages but implicit immune tolerance in selection is not one of them.\u00a0 &nbsp;&hellip;&nbsp;<a href=\"https:\/\/neoaptamers.com\/fr\/avoiding-self-recognition-with-aptamers\/\" rel=\"bookmark\">Lire la suite &raquo;<span class=\"screen-reader-text\">Comment nous \u00e9vitons l'autoreconnaissance avec les aptam\u00e8res<\/span><\/a><\/p>","protected":false},"author":2,"featured_media":3105,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"neve_meta_sidebar":"","neve_meta_container":"","neve_meta_enable_content_width":"off","neve_meta_content_width":70,"neve_meta_title_alignment":"","neve_meta_author_avatar":"","neve_post_elements_order":"","neve_meta_disable_header":"","neve_meta_disable_footer":"","neve_meta_disable_title":"","footnotes":""},"categories":[31,8,30,18],"tags":[],"class_list":["post-3101","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-advanced","category-aptamers","category-introductory","category-neomers"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>How We Avoid Self-Recognition with Aptamers | Neoaptamers.com<\/title>\n<meta name=\"description\" content=\"Learn about the 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