{"id":2973,"date":"2022-09-14T11:22:49","date_gmt":"2022-09-14T15:22:49","guid":{"rendered":"https:\/\/neoaptamers.com\/?p=2973"},"modified":"2022-11-24T22:43:08","modified_gmt":"2022-11-25T03:43:08","slug":"antibody-specificity","status":"publish","type":"post","link":"https:\/\/neoaptamers.com\/fr\/antibody-specificity\/","title":{"rendered":"Pourquoi les anticorps fonctionnent-ils mieux dans les matrices que les aptam\u00e8res ?"},"content":{"rendered":"<p><span style=\"font-weight: 400;\">Le grand gagnant de <a href=\"https:\/\/www.linkedin.com\/feed\/update\/urn:li:activity:6965313754079121408\/\">notre sondage sur la sp\u00e9cificit\u00e9 des anticorps<\/a> Le grand gagnant de notre sondage sur la sp\u00e9cificit\u00e9 des anticorps \u00e9tait que les anticorps ont \u00e9volu\u00e9 pour ignorer HAS\/IgG. Je lutte avec cela, car je pense que c'est trop simpliste. En r\u00e9alit\u00e9, la mani\u00e8re dont les anticorps se r\u00e9arrangent pour d\u00e9tecter les antig\u00e8nes \u00e9trangers est ouverte ; il n'y a pas de barri\u00e8res \u00e0 ce que les anticorps se d\u00e9veloppent contre les prot\u00e9ines du soi. Nous aurions probablement d\u00fb utiliser un terme diff\u00e9rent de \"Chckpt Inb\" (inhibition des points de contr\u00f4le) comme deuxi\u00e8me option, mais c'est l\u00e0 l'essence de pourquoi les anticorps fonctionnent bien dans les matrices. Les anticorps qui se d\u00e9veloppent pour se lier aux auto-antig\u00e8nes dans une matrice biologique, tels que l'albumine s\u00e9rique humaine ou les r\u00e9gions Fc des IgG, apparaissent sans aucun doute, mais ils sont \u00e9limin\u00e9s comme \u00e9tant dangereux. Il y a deux syst\u00e8mes, <strong>un guide<\/strong> \u2013 d\u00e9veloppement ouvert d'anticorps contre n'importe quelle cible, et un <strong>syst\u00e8me de contrebalancement<\/strong> qui d\u00e9tecte et \u00e9limine les anticorps qui se lient aux prot\u00e9ines du soi.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\">Ce syst\u00e8me de contrebalancement est ce qui manque \u00e0 la s\u00e9lection des aptam\u00e8res. Nous pouvons effectuer une contre-s\u00e9lection contre la matrice, mais cela \u00e9limine efficacement uniquement les aptam\u00e8res qui se lient tr\u00e8s fortement \u00e0 un composant de la matrice. La contre-s\u00e9lection contre l'albumine s\u00e9rique devrait th\u00e9oriquement \u00eatre r\u00e9alis\u00e9e \u00e0 la concentration \u00e0 laquelle l'albumine s\u00e9rique est pr\u00e9sente dans le sang (600 \u00b5M). Nous faisons cela en utilisant directement du s\u00e9rum dans la contre-s\u00e9lection avec notre <a href=\"https:\/\/neoaptamers.com\/fr\/frelex-advantages\/\">FRELEX<\/a> approche, ou en immobilisant toutes les prot\u00e9ines pr\u00e9sentes dans le sang sur de la r\u00e9sine UltraLink.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\">Notre nouveau <a href=\"https:\/\/neoaptamers.com\/fr\/the-neomer-selection-strategy-a-game-changing-innovation-from-neoventures\/\">plateforme de s\u00e9lection Neomer<\/a> repr\u00e9sente l'ajout d'un syst\u00e8me de contrebalancement pour \u00e9liminer tous les aptam\u00e8res qui se lient, m\u00eame faiblement, aux contre-cibles. Avec le syst\u00e8me Neomer, nous sommes en mesure de r\u00e9pertorier les s\u00e9quences d'aptam\u00e8res qui se lient aux contre-cibles dominantes comme l'HSA et les IgG, et de les \u00e9liminer en tant que s\u00e9quences candidates.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\">Pour en savoir plus sur la m\u00e9thode de s\u00e9lection d'aptam\u00e8res Neomer, veuillez :\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\"><a href=\"https:\/\/neoaptamers.com\/fr\/neomers\/\">Consultez notre conf\u00e9rence<\/a><\/span><\/p>\n<p><span style=\"font-weight: 400;\"><a href=\"https:\/\/neoaptamers.com\/fr\/contact-us\/\">Prenez contact sur notre site web<\/a><\/span><\/p>\n<p><span style=\"font-weight: 400;\">Ou contactez-nous par courriel \u00e0\u00a0<\/span><a href=\"mailto:info@neoventures.ca\"><span style=\"font-weight: 400;\">info@neoventures.ca<\/span><\/a><\/p>","protected":false},"excerpt":{"rendered":"<p>The clear winner of our poll on antibody specificity was that antibodies have evolved to ignore HAS\/IgG. I struggle with this, as I think it is too simple. Really, the way that antibodies rearrange to detect foreign antigens is open; there are no barriers to antibodies developing against self proteins. We probably should have used&hellip;&nbsp;<a href=\"https:\/\/neoaptamers.com\/fr\/antibody-specificity\/\" rel=\"bookmark\">Lire la suite &raquo;<span class=\"screen-reader-text\">Pourquoi les anticorps fonctionnent-ils mieux dans les matrices que les aptam\u00e8res ?<\/span><\/a><\/p>","protected":false},"author":2,"featured_media":2974,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"neve_meta_sidebar":"","neve_meta_container":"","neve_meta_enable_content_width":"off","neve_meta_content_width":70,"neve_meta_title_alignment":"","neve_meta_author_avatar":"","neve_post_elements_order":"","neve_meta_disable_header":"","neve_meta_disable_footer":"","neve_meta_disable_title":"","footnotes":""},"categories":[31,8],"tags":[],"class_list":["post-2973","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-advanced","category-aptamers"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Why Do Antibodies Perform Better in Matrices than Aptamers? 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